Over the past decade there has been an increased interest on the topic of “green” chemistry applied to chemical processes. These efforts aim at the total elimination or at least the minimization of generated waste and the implementation of sustainable processes through the adoption of 12 fundamental principles (which are: Atom Economy, Prevention, Less Hazardous Chemical Syntheses, Designing Safer Chemicals, Safer Solvents and Auxiliaries, Design for Energy Efficiency, Use of Renewable Feedstocks, Reduce Derivatives, Catalysis, Design for Degradation, Real-time Analysis for Pollution Prevention and Inherently Safer Chemistry for Accident Prevention). Any attempt to reach these goals must comprehensively address these principles in the design of a synthetic route, chemical analysis, or chemical process. Utilization of nontoxic chemicals, environmentally acceptable solvents, and renewable materials are some of the key issues that merit important considerations in a green synthetic strategy. In the present work, we present a totally green approach toward the synthesis and stabilization of metal nanoparticles. Produced nanoparticles will be investigated for their applicability in biomedical applications especially in cancer therapy. In our study, we will focus on finding a novel green synthesis routes for silver nanoparticles (AgNPs) using different extracts from a variety of medicinal plants. The aim of this study is the investigation of the anti-cancer activities and antiproliferative activities of the bioactive AgNPs along with their capping biomolecules in vitro. Prerequisite for every possible application is the proper surface functionalization of these nanoparticles, which determines their interaction with the environment (such as cancer cells), so one focus of this research study is the development of surface modification strategies and functionalization of silver colloidal nanoparticles for later in vivo applications. Especially the study of functionalization of silver nanoparticles with biomolecules still needs intensive studies. So we will try to achieve mechanistic insights by careful characterization of produced nanoparticles. Therefore, the obtained product in each stage of the work will be characterized by X-ray diffraction (XRD), energy dispersive X-ray (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible absorption spectroscopy, dynamic light scattering (DLS), and zeta potential measurement.